Ikaros C-terminal (Source: Smale lab, Reference: Hahm et al., 1998)
replicate
ICN1 (Ik-C Ab)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde at room temperature for 10 min and Ikaros ChIP were performed with two different Ikaros Antibodies using protocol essentially as descrbed in O'Geen, H., Frietze, S. & Farnham, P.J. Using ChIP-seq technology to identify targets of zinc finger transcription factors. Methods Mol. Biol. 649, 437–455 (2010) Single-end multiplexed libraries were created essentially as described in O'Geen, H., Frietze, S. & Farnham, P.J. Using ChIP-seq technology to identify targets of zinc finger transcription factors. Methods Mol. Biol. 649, 437–455 (2010)